polyclonal rabbit anti bdnf  (Bioss)

Journal: Open Life Sciences

Article Title: Resveratrol modulates FABP5 to reduce neuronal apoptosis following ischemic stroke

doi: 10.1515/biol-2025-1253

Figure Lengend Snippet: Comparison of BDNF, NSE, and FABP5 levels in brain tissues of rats across groups. (A) Western blot analysis of BDNF, NSE, and FABP5 protein expression in the brain tissues of each group. (B) Immunofluorescence detection of FABP5 in brain tissues of each group. Green fluorescence indicates FABP5 protein expression. (C–E) Western blot and relative quantification of NSE, FABP5, and BDNF expression levels in each group. (F–H) Statistical analysis of mRNA levels of NSE, FABP5, and BDNF in each group.

Article Snippet: Further, radioimmunoprecipitation assay lysis buffer (ES-8150; Ecotop, China), bicinchoninic acid protein assay kit (EK-5001; Ecotop), polyvinylidene difluoride membrane (ISEQ00010; Millipore, USA), AMPKa1/AMPKa2 mouse mAb (1:1000, A27099 , ABclonal, China), BDNF polyclonal (1:1000, 28205-1-AP; Proteintech, China), FABP4 (1:2000, ab92501; Abcam, England), FABP5 (1:1000, ab151772; Abcam, and FABP3) (1:800, A8789; ABclonal) were used in the study.

Techniques: Comparison, Western Blot, Expressing, Immunofluorescence, Fluorescence, Quantitative Proteomics

Journal: Journal of Translational Medicine

Article Title: Intranasally administered muse cells attenuate neurodegeneration in Parkinson’s disease

doi: 10.1186/s12967-025-07401-6

Figure Lengend Snippet: Muse cell has protective effects on DA neurons. A : (200×magnification) Compared to normal WT mice, the PBS group showed reduced TH and NeuN staining in the SNc region. In contrast to the PBS group, the muse group exhibited a higher number of TH-positive cells and NeuN-positive cells, with PKH26-labeled muse cells present in the region. Compared to the muse group, the muse + J and non-muse groups displayed relatively fewer TH-positive cells( n = 6). B : S1P expression in the brain tissue of A53T mice (PBS group) was significantly increased compared with normal WT mice. In the group treated with muse cells, S1P expression was markedly reduced relative to both the PBS and non-muse groups, though it remained higher than that in normal WT mice. Western blot results indicated: TNF-α expression in the brains of the PBS group was significantly higher than in control. Compared to the PBS group, TNF-α expression was significantly reduced in the muse group, while the non-muse and muse + J groups also showed reduced TNF-α expression. TNF-α expression in the muse group was lower than in the non-muse and muse + J groups. Expression levels of BDNF and GDNF showed a marked decline in the PBS group. No significant change was seen with non-muse cell treatment, whereas the muse group displayed elevated expression. The expression remained unchanged in the muse + J group. (*Compared to the control group, ** P < 0.01, and compared to the PBS group, # P < 0.05, ## P < 0.01, & compared to the muse group, & P < 0.05) ( n = 3). C : The H&E staining results (200× magnification) showed that in the substantia nigra region of normal WT mice, the tissue structure was normal, and the cell nuclei were clearly visible (red arrows indicate astrocytes, green arrows indicate neuronal cells). In contrast, the brains of PBS group mice exhibited significant neuronal loss, with abnormal neuronal nuclear morphology and blurred boundaries. The non-muse group and muse + J group showed similar conditions to the PBS group, but the degree of neuronal damage was reduced. In the muse group, the number of damaged neurons was significantly decreased, and the tissue characteristics were similar to those of normal WT mice. (red→astrocyte, green→neuronal cells, black→damaged neuronal cells) ( n = 6)

Article Snippet: BDNF Polyclonal antibody , 28205-1-AP , Proteintech.

Techniques: Staining, Labeling, Expressing, Western Blot, Control